mouse tnf α paired antibodies  (R&D Systems)

Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

doi: 10.1016/j.ijpddr.2026.100642

Figure Lengend Snippet: Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.

Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

Techniques: Control, Migration

Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

doi: 10.1016/j.ijpddr.2026.100642

Figure Lengend Snippet: Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).

Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

Techniques: Activity Assay, Control